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70k tkov3 library  (Addgene inc)


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    Addgene inc 70k tkov3 library
    70k Tkov3 Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/toronto+knockout+version+3+tkov3+crispr+knockout+library/Toronto+KnockOut+(TKO)+CRISPR+Library+-+Version+3+(Pooled+Libraries+%2390294%2C+%23125517)/bio_rxiv__64898__2026__03__16__712096-331-26-29
    Average 94 stars, based on 92 article reviews
    70k tkov3 library - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Genome-Wide CRISPR Screen Reveals PIK3CA Inhibition Enhances Lipid Nanoparticle-Mediated siRNA Delivery.
    Article Snippet: .. Lentiviral Production: The pooled plasmid of the Toronto KnockOut version 3 (TKOv3) CRISPR knockout library was obtained from Addgene (#90 294). .. Lentiviral particles were produced using Lenti-X 293T cells (632 180, Takara Bio).

    Article Title: Genome‐Wide CRISPR Screen Reveals PIK3CA Inhibition Enhances Lipid Nanoparticle‐Mediated siRNA Delivery
    Article Snippet: .. The pooled plasmid of the Toronto KnockOut version 3 (TKOv3) CRISPR knockout library was obtained from Addgene (#90 294). .. Lentiviral particles were produced using Lenti‐X 293T cells (632 180, Takara Bio).

    Knock-Out:

    Article Title: Genome-Wide CRISPR Screen Reveals PIK3CA Inhibition Enhances Lipid Nanoparticle-Mediated siRNA Delivery.
    Article Snippet: .. Lentiviral Production: The pooled plasmid of the Toronto KnockOut version 3 (TKOv3) CRISPR knockout library was obtained from Addgene (#90 294). .. Lentiviral particles were produced using Lenti-X 293T cells (632 180, Takara Bio).

    Article Title: Genome‐Wide CRISPR Screen Reveals PIK3CA Inhibition Enhances Lipid Nanoparticle‐Mediated siRNA Delivery
    Article Snippet: .. The pooled plasmid of the Toronto KnockOut version 3 (TKOv3) CRISPR knockout library was obtained from Addgene (#90 294). .. Lentiviral particles were produced using Lenti‐X 293T cells (632 180, Takara Bio).

    CRISPR:

    Article Title: Genome-Wide CRISPR Screen Reveals PIK3CA Inhibition Enhances Lipid Nanoparticle-Mediated siRNA Delivery.
    Article Snippet: .. Lentiviral Production: The pooled plasmid of the Toronto KnockOut version 3 (TKOv3) CRISPR knockout library was obtained from Addgene (#90 294). .. Lentiviral particles were produced using Lenti-X 293T cells (632 180, Takara Bio).

    Article Title: Genome‐Wide CRISPR Screen Reveals PIK3CA Inhibition Enhances Lipid Nanoparticle‐Mediated siRNA Delivery
    Article Snippet: .. The pooled plasmid of the Toronto KnockOut version 3 (TKOv3) CRISPR knockout library was obtained from Addgene (#90 294). .. Lentiviral particles were produced using Lenti‐X 293T cells (632 180, Takara Bio).



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    (A) Schematic representation of the screening protocol. HEK293T cells expressing GFP-Parkin R42P from the landing pad were transduced with the <t>TKOv3</t> sgRNA library. Fluorescence activated cells sorting (FACS) was used to collect the cells with the 5% highest and lowest GFP:mCherry ratios. Finally, the sgRNAs were identified by sequencing. Created in BioRender. Hartmann-Petersen, R. (2025) https://BioRender.com/mxx8owh . (B) Plot displaying sgRNA enrichment in the 5% highest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green. (C) Plot displaying sgRNA enrichment in the 5% lowest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green.
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    (A) Schematic representation of the screening protocol. HEK293T cells expressing GFP-Parkin R42P from the landing pad were transduced with the <t>TKOv3</t> sgRNA library. Fluorescence activated cells sorting (FACS) was used to collect the cells with the 5% highest and lowest GFP:mCherry ratios. Finally, the sgRNAs were identified by sequencing. Created in BioRender. Hartmann-Petersen, R. (2025) https://BioRender.com/mxx8owh . (B) Plot displaying sgRNA enrichment in the 5% highest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green. (C) Plot displaying sgRNA enrichment in the 5% lowest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green.
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    (A) Schematic representation of the screening protocol. HEK293T cells expressing GFP-Parkin R42P from the landing pad were transduced with the <t>TKOv3</t> sgRNA library. Fluorescence activated cells sorting (FACS) was used to collect the cells with the 5% highest and lowest GFP:mCherry ratios. Finally, the sgRNAs were identified by sequencing. Created in BioRender. Hartmann-Petersen, R. (2025) https://BioRender.com/mxx8owh . (B) Plot displaying sgRNA enrichment in the 5% highest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green. (C) Plot displaying sgRNA enrichment in the 5% lowest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green.
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    Image Search Results


    (A) Schematic representation of the screening protocol. HEK293T cells expressing GFP-Parkin R42P from the landing pad were transduced with the TKOv3 sgRNA library. Fluorescence activated cells sorting (FACS) was used to collect the cells with the 5% highest and lowest GFP:mCherry ratios. Finally, the sgRNAs were identified by sequencing. Created in BioRender. Hartmann-Petersen, R. (2025) https://BioRender.com/mxx8owh . (B) Plot displaying sgRNA enrichment in the 5% highest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green. (C) Plot displaying sgRNA enrichment in the 5% lowest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green.

    Journal: bioRxiv

    Article Title: BAG6 and RNF126 are broadly involved in protein quality control of non-native missense protein variants

    doi: 10.64898/2026.02.04.703735

    Figure Lengend Snippet: (A) Schematic representation of the screening protocol. HEK293T cells expressing GFP-Parkin R42P from the landing pad were transduced with the TKOv3 sgRNA library. Fluorescence activated cells sorting (FACS) was used to collect the cells with the 5% highest and lowest GFP:mCherry ratios. Finally, the sgRNAs were identified by sequencing. Created in BioRender. Hartmann-Petersen, R. (2025) https://BioRender.com/mxx8owh . (B) Plot displaying sgRNA enrichment in the 5% highest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green. (C) Plot displaying sgRNA enrichment in the 5% lowest GFP:mCherry bin. Significant (p < 0.001 and log2 fold change > 0.5) hits are colored red. Control sgRNAs are marked in green.

    Article Snippet: The Toronto human knockout pooled library (TKOv3) (Addgene #90294) , psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) were from Addgene.

    Techniques: Expressing, Transduction, Fluorescence, Sequencing, Control